High Performance Liquid Chromotography (HPLC)

A general term applied to modern instrumental techniques adopted to greatly increase the scope and precision of the liquid chromatography analytical method.

Among the varied HPLC techniques are:

Reversed phase chromatography, which uses a polar liquid phase for elution of a column containing a nonpolar phase. This technique is used, for example, to analyze the polynuclear aromatic hydrocarbon category (pyrenes, naphthalenes, etc.) of synthetic organic compounds; Ion exchange chromatography, which makes it possible to separate large numbers of cations and anions; Exclusion chromatography, which separates, on a porous gel, compounds according to their size and makes it possible to determine their apparent molecular weights and to separate fractions of different molecular weights for further analyses.

See Also: Chromatography Liquid Chromatography Gas Chromatograph (GC) Gas Chromatography (GC) Mass Spectrometry (MS) Chromatography Liquid Chromatography Chromatography Liquid Chromatography Gas Chromatograph (GC) Gas Chromatography (GC) Mass Spectrometry (MS)